- Male: 24
- Female: 10
- Neither/Unknown: 3
*Counting characters of unknown gender (e.g., Pokémon) as neither, characters where you can choose the gender as both, and alternate versions of characters that the game treats as separate characters (e.g. Link and Toon Link, Samus and Zero Suit Samus) as two separate characters. Also it's kind of the whole point of Sheik that he has a masculine gender presentation (with some ambiguity as to whether the transformation alters him physiologically or cosmetically, but either way, it's masculine presentation), so I'm counting him as male.
ETA: Corrected a math error. 10/37 is a bit over 1/4, not a bit over 1/3.